Kyiv-Lviv flights will be operated on Thursdays and Sundays until October 26, after which the number of flights will increase to four per week.
The aircraft has already landed in Ukraine.
The airline has obtained the relevant permission from the State Aviation Administration.
The airline performed more than 5,200 flights from May 2018 to May 2019.
The aircraft arrived at the Zhulyany airport on November 29.
The Boeing 737-800, which was manufactured in 2013, was issued a Ukrainian tail number on November 22.
The flights will begin in June 2019.
The airline is awaiting delivery of two more Boeing aircraft this month.
The airline took delivery of two Boeing 737-700 airliners and one Boeing 737-800 airliner.
SkyUp is preparing to announce new routes to Europe.
The airline currently operates only one Boeing 737-800 airliner.
The airline plans to operate three regular flights from Odesa to Kyiv, Khrarkiv, Lviv.
The first flight was performed to Egypt.
SkyUp will initially operate international charter flights
Boeing 737-800 is 4.7 years old.
SkyUp Airlines is the first airline to place an order for Boeing 737 MAX 10 aircraft not only in Ukraine
SkyUp will start with four aircrafts.
The airline will operate charter flights from Kyiv, Kharkiv, Lviv, and Odesa to 16 destinations